Evidence and limitations

Test the workflow.
Review the biology.

Execution, file compatibility and biological accuracy are separate questions.

Local model runs

Recorded device: NVIDIA GeForce RTX 3060. PyTorch 2.11.0+cu126; CUDA runtime 12.6.

Docker release checks: 39 container tests and 15 offline public-data cases passed on Linux/amd64, including CPU, CUDA, all three models and both movie outputs. See the Docker validation record for exact image digests and measured results.

DatasetModelModeObjectsRuntimePeak GPU allocationResult
Fluo-N2DH-GOWT1general_2dgreedy1381.04 s52 MiBPassed
Fluo-N2DH-SIM+general_2dgreedy1800.73 s52 MiBPassed
PhC-C2DH-U373general_2dgreedy360.42 s51 MiBPassed
Fluo-N2DH-GOWT1ctcgreedy1380.73 s116 MiBPassed
Fluo-N2DH-SIM+ctcgreedy1800.58 s117 MiBPassed
PhC-C2DH-U373ctcgreedy360.48 s115 MiBPassed
Fluo-N2DH-GOWT1general_2d_w_SAM2_featuresgreedy1383.04 s729 MiBPassed
Fluo-N2DH-SIM+general_2d_w_SAM2_featuresgreedy1802.09 s730 MiBPassed
PhC-C2DH-U373general_2d_w_SAM2_featuresgreedy361.94 s730 MiBPassed
Fluo-N2DH-SIM+-01-65frames.ome.zarrgeneral_2dgreedy26075.84 s54 MiBPassed
Fluo-N2DH-GOWT1-01-92frames.ome.zarrgeneral_2dgreedy20589.89 s52 MiBPassed

Each listed run loaded verified local weights. Validation compares output pixels and original labels with input, checks SQLite integrity and retains model/dataset hashes. Tracking-stage runtime includes copying, inference and table preparation; movie rendering and final export are excluded. GPU figures are PyTorch peak allocation, not total device usage.

Automated coverage

Axis reordering, anisotropic coordinates, ordered transforms, calibration rejection, mismatched grids, HCS isolation, source preservation, overwrite refusal, memory guards, CLI types, checkpoint integrity, gaps, divisions, empty inputs and failed-run publication are tested. See GitHub Actions and the local validation record.

Practical limits

Reproduce

test.cmd
.venv\Scripts\python tools\prepare_publicdata.py
.venv\Scripts\python tools\validate_local.py --device cuda
.venv\Scripts\python tools\build_site.py --validation test-results/public/validation.json
.venv\Scripts\python tools\check_site.py docs